mab6495  (R&D Systems)

Journal: Cancer Science

Article Title: Ubiquitin C‐terminal hydrolase‐L1 has prognostic relevance and is a therapeutic target for high‐grade neuroendocrine lung cancers

doi: 10.1111/cas.14284

Figure Lengend Snippet: Protein expression of UCHL1, p53 and RB1, and mRNA expression of UCHL1 in various lung cancer cell lines and normal lung epithelial cells. A, Increased UCHL1 levels in all small cell lung cancers (SCLC) tested (H82, H69 and H526) and in three non–small cell lung cancer (NSCLC) cell lines (A549, H1299 and PC9) was confirmed by western blotting. B, Comparison of UCHL1/β‐actin protein expression ratio among the cell lines showed that SCLC consistently had high UCHL1 levels, whereas NSCLC showed different expression patterns depending on the cell line. C, Comparison of UCHL1 mRNA expression among the cell lines showed that all lung cancer cell lines except for HCC827 had high UCHL1 levels

Article Snippet: The following were used as primary antibodies: UCHL1 (#13179; CST), p53 (#9282; CST), RB1 (MAB6495; R&D Systems), β‐actin (A5316; Sigma‐Aldrich), CD9 (EXOAB‐CD9A‐1; SBI) and CD63 (EXOAB‐CD63A‐1; SBI).

Techniques: Expressing, Western Blot, Comparison

Journal: BMC Biotechnology

Article Title: Simultaneous silencing of multiple RB and p53 pathway members induces cell cycle reentry in intact human pancreatic islets

doi: 10.1186/1472-6750-14-86

Figure Lengend Snippet: Simultaneous knockdown of multiple genes in primary human islets following electroporation. (A) Graph shows RT-qPCR results of islets electroporated with non-targeting control siRNA or siRNA targeting RB and p130 (L-003299). (B) Graph shows RT-qPCR results of islets electroporated with non-targeted control siRNA or siRNAs targeting the indicated gene products. The housekeeping gene GAPDH was used as the control. Representative experiment shown from 3 separate experiments. Error bars indicate standard deviation. (C) Western blot for RB protein in human islet lysates after electroporation of control or targeting siRNA. GAPDH was used as a loading control. RB appears as a characteristic doublet representing the hypo and hyperphosphorylated protein. Representative experiment shown from 3 different experiments.

Article Snippet: For detection of Retinoblastoma protein blots were probed with 0.1 μg/mL of Human RB1 (MAB6495, R&D Systems) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (R &D Systems).

Techniques: Knockdown, Electroporation, Quantitative RT-PCR, Control, Standard Deviation, Western Blot